(C’) Histological section (plane marked in (C)). (C) Expression in the gastrocoel roof plate (GRP) at stage 17 (dorsal explant shown in ventral view). Note that the superficial mesoderm (SM) was free of PACRG mRNA (inset in (B’)). (B’) Sagittal hemisection (plane indicated in (B)) revealing mRNA localization in deep mesoderm. Persistent differences in staining intensities between dorsal and ventral side. Enlargements (boxes) indicate higher expression levels on the dorsal side. (A’) Sagittal hemisection of embryo shown in (A).
(A) Expression at the four-cell stage (top view). PACRG expression during early Xenopus development.
